A Detailed Guide on Magic Mushroom Spore Syringes (2024)

If growing mushrooms is important to you, knowing how to make a spore syringe is an essential skill to learn. Growing mushrooms is a long, tedious, but rewarding process, with many detail oriented steps.

One of the first tasks in your journey is to inoculate a growing medium with mushroom genes. Inoculation can be performed using several methods, although using a spore syringe is considered one of the easiest and cleanest options, making spore syringes a desirable choice for beginners.

A spore syringe is a syringe filled with sterile water and hydrated mushroom spores. Mushroom spores are the reproductive microscopic cells of fungi. They are the propagative element of a mushroom, and they enable the mushroom to reproduce and disperse itself.

Spore syringes are used in the mycology space to begin the mushroom life cycle, by inoculating a growing medium. Furthermore spores can be used to continue a particular mushroom’s genetic line from your current crop to your next crop.

Spore syringes are praised by mycologists of all experience levels, but they are especially helpful for beginners because of how easy they are to use. Choosing the spore syringe germination method can actually reduce the chances of contamination during inoculation when compared to other methods of germination such as agar.

Because spores within a syringe do not have any contact with the open air, the contamination risk during inoculation is greatly reduced. Although we still highly recommend performing any spore tasks in front of a ow hood or inside a still air box.

In order to make a spore syringe you’ll first have to obtain clean mushroom spores. For this guide we recommend getting your spores from a mushroom spore print. Spore prints can be sourced online, although make sure you are buying from a reputable source who carries clean prints.

Alternatively if you’re already growing mushrooms you could make your own spore print, see our guide below to learn how.

Read: Make your Own Spore Prints

Before you start you will need to source some supplies such as spores and syringes. Since we have already touched on spores above let’s get into syringes. Syringes can be sourced from your local pharmacies for a hefty price, or can be ordered in bulk online. If you become friendly with your local pharmacist they may be able to order bulk syringes for you at a discounted price. Be-sure to obtain luer-lock syringes as they have a screw-like ending where you can attach a needle or a cap for protection during transport.

One more thing, size matters. Syringes come in different sizes, you can source whichever size you want but 10 ml is the standard size for mycology. To produce a successful spore syringe, one must ensure their workspace is as clean as possible. Making a spore syringe in open free owing air will result in contamination of the spores from other fungi spores and bacteria present in the air. I highly recommend you do your work in front of a laminar flow hood, if not then at the very least inside of a still air box.

Furthermore, be sure to wipe down all of your tools and equipment with 70% isopropyl alcohol and use same sterilization when necessary. Finally organize all of your tools in a neat manner and place them within reaching distance as well as inform your housemates of the task at hand and politely ask them not disturb your sterile environment.

Equipment:

• Sterile syringe (luer lock, 10ml + preferred)
• Sterile needle (blunt tip is best for sucking up spores)
• Syringe cap for storage, or normal needle for inoculation
• Sterile water
• Drinking glass or shot glass
• Still air box (SAB) or laminar flow hood
• PPE (gloves, masks)
• 70% Isopropyl Alcohol
• Gas lighter or flame torch
• Pressure cooker
• Tweezers Scalpel
• Spore print

Allow the water and other contents inside the pressure cooker to cool. Do not rush this step, if the water is too hot it will kill the spores in the later steps.

The cooling process can take several hours or even overnight. Avoid opening the pressure cooker until you are ready to work, as doing so could potentially expose the contents to contaminants.

Now comes the fun part! Sterilize your workspace especially the tweezers and scalpel, you may use a flame for these tools.

With the spores now fully hydrated, the syringe is either ready for use or it can be stored in the fridge for later use.

Using a spore syringe is often referred to as “inoculation”, which basically means implanting a microbe into another organism. In this case you will be injecting the spores into a growing medium such as sterilized grain spawn. It is of the utmost importance to ensure the mushroom spawn is sterilized properly, and to clean the point of injection, as well as the tip of the needle before inoculation.

To inoculate mushroom spawn with a spore syringe, begin by allowing the syringe to reach room temperature. Shake the syringe well before injecting some or all of the spore solution into the spawn medium. After spore injection, it’s important to seal off the injection area to prevent unwanted mold and other fungi species from competing with the mushrooms that you are trying to grow.

Inoculating by liquid culture is another popular method of mushroom germination in the mycology world. Essentially spores begin growing mycelium within a nutrient enriched sterile water solution versus that of a spore syringe, where the spores are injected into a growing medium for mycelium to colonize.

Colonization can be quicker with liquid culture, since the mycelium has already started growing, although unless you can verify that the liquid culture came from a single isolated strain, you may run into several problems. Further into the growing process you might experience genetic competition and weak genes resulting in stunted growth.

Read: Grow your own Mushrooms (Step by Step)