Sterilized Substrate | Mushroom Substrate Bags | Sterile Substrates - Myterra Labs (2024)

FAQs

How do you store sterile substrates? ›

Sterile substrates do not require refrigeration, we recommend storing at room temperature. If kept at room temperature and out of direct sunlight, all sterile substrates can be stored for several months before use.

Generally, sterilizing substrate blocks in a pressure cooker at 250°F (121°C) takes anywhere from 1 to 4 hours. The minimum sterilization time for small blocks of substrate is one hour, and for large blocks, 3 to 4 hours. If you leave the blocks for too long, there's a danger of over-sterilization.

Store your 50/50 substrate in a cool and dry location. 50/50 substrate can keep for up to 4 months in these conditions. There will be no need to rehydrated or sterilize the products a second time. If you need to store the product longer it can be refrigerated for up to 6 months and frozen for up to 1 year.

Achieving a substrate sterilization requires a temperature of at least 250°F (121°C) for a minimum of 2 hours, which cannot be accomplished by boiling water or steam alone.

You don't always need to sterilise or pasteurize the mushroom substrate. This simply minimises the risk of contamination-related growth issues or sickness. Some mushrooms cannot be cultivated on sterile media and must instead be grown outdoors or in nutrient-rich environments that do not allow for sterilisation.

Sterile items that become wet are considered contaminated because moisture brings with it microorganisms from the air and surfaces. Closed or covered cabinets are ideal but open shelving may be used for storage.

Storage of Sterile Items

All sterile packs must be labeled with the date of autoclaving. They should be stored in a closed cabinet and protected from moisture. As a general rule, packs wrapped in cloth or woven paper should be used within 1 month of autoclaving and sealed peel packs should be used within 1 year.

Pasteurization will not sterilize—or remove all contaminants—the way actual sterilization will. It really only weakens microorganisms, killing some pathogenic bacteria and reducing the level of substrate contaminants in the process.

The method of ste- rilization with hydrogen peroxide H2O2 can be used as a successful method for sterilization of cultivation substrates for oyster mushrooms, taking into account the soaking for a longer period and increasing the period of time before ad- ding the mushroom spawn to the substrates to get rid of the ...

To use the steam method, bag the substrate in filter patch bags and cook it in a pressure cooker or steam it until it reaches around 160-180 degrees F. Allow your bulk substrate to cool completely before using it!

Where do you store mushroom grow bags? ›

All you need to do is keep your bag in a warm place inside your house. Between 20 to 24 C is an ideal temperature range. Luckily for most people, that's also around the ambient temperature inside your home.

Hot Water Pasteurization

The hot water at this temperature is effective in killing heat-sensitive competitors while leaving some beneficial heat-tolerant bacteria alive in the substrate. It is important to be mindful of the water temperature, as overheating can kill the beneficial bacteria.

We recommend storage temperature to be 34-42°F. Refrigeration at warmer than recommended temperatures will shorten shelf life and may put spawn at risk of fruiting in the bag which can affect the way the spawn handles when using it. PLEASE NOTE: Pink Oyster and Almond Agaricus spawn CANNOT be refrigerated.

Place the grain bags or quart jars in your pressure cooker to sterilize based on the method used (bag or jar) and the size of the bag. The recommended pressure is 15 PSI. Once sterilized in the pressure cooker, you want your spawn grain to cool at room temperature typically for 12+ hours.

In the United States and several other countries, ethylene oxide (ETO) gas is used for sterilization of heat- and moisture-sensitive surgical instruments, such as plastic devices and delicate instruments.

A sterilization pouch, or peel pack, is a disposable package used in a sterilizer to allow penetration of the sterilant to the items placed inside. After sterilization, these Class II Medical Devices maintain the sterility of the processed item.