Understanding Mushroom Substrates - FreshCap Mushrooms (2024)

Finding the perfect mushroom substrate for your grow-and getting the right substrate recipe– is one of the most important aspects of mushroom cultivation.

New growers will quickly realize that growing mushrooms is significantly different than growing plants.

Although the concept of a mushroom substrate can be thought of as somewhat analogous to the soil for a plant, the way that mushrooms actually use a substrate is different, and needs to be fully understood by the cultivator in order to achieve the best results.

Common Mushroom Substrates


Pasteurized Straw is commonly used to grow oyster mushrooms. It is cheap and effective, albeit a little messy.

Hardwood Sawdust

Hardwoods such as oak, beech and maple make for a great substrate for many types of mushrooms, especially when combined with a bran supplement.

Soy Hulls

Soy hulls can be mixed with hardwood sawdust at different ratios to produce extremely effective and high yielding substrates, particularly for growing oysters.


Common button mushrooms are grown on composted manure, specially prepared using a two phase composting and sterilization process.

Coco Coir and Vermiculite

Coir is a commercially available mix of ground up coconut husk and shells which can be mixed with vermiculite to create an effective substrate.

Mushrooms will grow on a number of urban and agricultural waste products, from spent coffee grounds to banana leaves.

What is a Mushroom Substrate?

To put it simply, a substrate is a bulk material that the mushroom mycelium can use for energy and nutrition. A good substrate is required in order for the mushroom to grow and to fruit.

Common substrate recipes for growing mushrooms usually involve straw or hardwood sawdust, although there are many other good options to be considered.

The mushroom substrate needs to be properly prepared by adding water, potentially adding additional nutrition, and further processed by sterilization or pasteurization. When ready, it can be “Innoculated”, wherein broken up pieces of mycelium-covered grain, also known asgrain spawn, are evenly mixed into the substrate.

Under the right conditions, the mycelium will start to grow, rapidly devouring and decomposing the organic material in a process known as “colonization.”

The mushrooms are ready to fruit once the substrate is fully colonized- meaning it has been completely consolidated and encompassed by the mycelium.

Understanding Mushroom Substrates - FreshCap Mushrooms (1)

Pink Oysters Pinning on a Fully Colonized Straw Based Substrate.

Pasteurizing or Sterilizing the Substrate

Ideal mushroom substrates are moist and full of nutrition. Unfortunately, there are many other things that also thrive in these conditions, such as molds and bacteria. These contaminants can grow much faster than the mushroom mycelium, so something needs to be done in order to give the mushroom an advantage in the race to colonize the substrate.

This can be accomplished by the process ofsterilizationorpasteurization.

Pasteurization is the process of heating up a substrate between 150-180 deg F for a period of 1.5-2 hours. It doesn’t removeallthe contaminants, but it will reduce the overall population of other microbes to a level that gives the mushroom species a head start.

Some microorganisms will survive pasteurization and remain in the substrate in some capacity, but can actually helpful for the growth of the mushroom in the long run.

Using Straw as a Substrate

Straw is a cheap and extremely effective substrate that works well for many types of mushroom species. Straw is thestemportion of grains such as wheat, barley or oats – it’s basically what is left over after the grain itself has been removed.

In many ways, straw is an agricultural waste product.

If you live near a farming community, you should have no problem finding straw. A 40lb (dry weight) square bail should cost no more than $2-4. Keep in mind that straw is messy to work with – it needs to bechopped, cleaned and pasteurizedbefore being used as a substrate. It works best for medium to large grow operations which have a separate prep area and a large drum for pasteurization.

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A 40lb square bail of straw, compressed.

That being said, you can still use straw for hobby scale indoor grows. A smaller amount of straw can be chopped with garden shears and pasteurized by pouring hot water into a tote- instead of using a large drum and a gas burner.

If you are planning to do small scale grows using straw, you might want to consider usingEZ Straw. This is especially convenient if you don’t live anywhere near a farming community. They sell coarsely cut straw in a convenient bag, already pre-cut and cleaned. Using something like EZ Straw will prevent a lot of the mess and hassle of dealing with large bales of straw.

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Using Hardwood Sawdust and Chips

Hardwood sawdust makes for an excellent mushroom substrate, and is commonly used in industry. It is a waste product of the lumber industry, and might be cheap and easy to find, depending on your location.

The fine sawdust is typically mixed with wood chips, which seems to allow for faster colonization of the substrate and provide a better structure for the mycelium to take hold.

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Commonly used hardwoods are oak, maple, beech, and hickory- or a mix of multiple species. Softwoods such as spruce, pine and fir are not suitable for mushroom cultivation.

If you are in an area where you don’t have easy access to hardwood sawdust, you can always use hardwood pellets. These are typically used for wood stoves- but will also work well for growing mushrooms! The pellets need to be soaked in water before being broken up into fine sawdust\. Soaking in water will also serve to hydrate the substrate.

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You can usually find the pellets inexpensively, although they may only available seasonally in most retail stores. There is a large selection of them availablehere.

They usually come in large 40lb bags, which should be enough sawdust for most small scale hobby grows. Again, make sure the pellets are hardwood and not softwood. You also want to make sure that the pellets don’t contain a lot of glues, paints or other additives.

If you are growing mushrooms using sawdust pellets, the following substrate recipe works well for many types of gourmet mushrooms:

For every 5 lb fruiting block:

  • 5 cups of hardwood pellets
  • 1.4 liters water
  • 1-1/4 cups wheat bran

Check out our article ongrowing mushrooms on supplemented sawdust fruiting blocksfor more information.


Mushroom substrates can supercharged by adding an easily consumed and highly nutritious supplement that the mushroom mycelium can use to grow faster and stronger.

Supplementation will also produce larger yields! Be careful though, as too much supplementation can cause higher rates of contamination.

Typically, supplementation is achieved by adding bran (oat bran or wheat bran) at a ratio of 5-10% dry weight. Substrates that are supplemented absolutely need to be fully sterilized or else contaminants will quickly take over.

Even after full sterilization, the chance of contamination goes up linearly with the amount of supplementation- and eventually you reach a point of diminishing returns.

Interestingly, Shiitake mushrooms require lower levels of supplementation in order to produce the best results. Over-supplemented shiitake mushrooms can produce strange and unsellable mutated looking fruiting bodies.

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Shiitake Naturally Growing on Hardwood Logs.

The Masters Mix

If you’re looking for anextremelyeffective substrate for growing oysters, you might want to try what’s know as theMaster’s Mix– a 50/50 mix of sawdust and soyhulls, sterilized at 15 PSI for 2.5 hours.

The original “master” behind this mix is T.R. Davis from Earth Angel Mushrooms. You will not likely find any another substrate that will yield as much on the first flush as this mix.

There are two downsides to using soy hulls as far as I can tell. Firstly, the second flush will not be anywhere nearly as strong as the first flush, even though thehugefirst flush definitely makes up for it.

Secondly, the mix doesn’t seem to colonize as fast as the more traditional supplemented sawdust formula. It’s possible that this is only in my experience, so your miles may vary.

Growing Mushrooms on Manure

Most people think that mushrooms grow on manure- which is indeed true. Although most gourmet species prefer hardwood sawdust or straw, there are many common species that grow best on manure.

Agaricus species, such as the common button mushroom and the portobella mushroom, as well as many Psilocybin species, are good examples.

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In commercialAgaricus bisporuscultivation, the manure substrate first goes through a multiday composting phase, where large piles of compost (manure and straw) naturally heat up to 160 deg F, and beneficial organisms proliferate.

Once this process is complete, the compost is properly pasteurized to remove all unwanted contaminants. This secondary pasteurization process also removes ammonia that naturally builds up during the first phase compost. Check out this neat video below that sums up the process of preparing compost for button mushroom cultivation:

Growing mushrooms on compost in this manner is usually not pursued by the home grower, though it is certainly possible.

Growing Mushrooms on Coco-Coir

Another common material used to grow mushrooms at home is coco-coir. Coco-coir is a natural material made from the husk and the shell of coconuts.

It is commonly used for quasi-hydroponic plant growing systems. It does a good job of retaining water, and although it doesn’t provide many nutrients for plants, it is reasonably nutritious enough for growing mushrooms, although not so much so that contaminates easily proliferate.

Coco coir is often mixed in a 1:1 ratio withvermiculiteto form a suitable mushroom substrate. Vermiculite is an expanded mineral that provides structure to the substrate and retains water, though it is nutritionally inert. The coco-coir vermiculite mix still needs to be properly pasteurized before being inoculated with mushroom grain spawn.

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You can buy compressed blocks of coco-coir onAmazon, and can also usually find it at your local garden center. The coco-coir will expand many times it’s size once it is broken apart and soaked with water.

Growing Mushrooms on Coffee

Although it is possible to use nothing but spent coffee grounds as a mushroom growing substrate, it is usually not the best choice. Coffee grounds are high in nitrogen, which provides plenty of energy for the mycelium and helps produce higher yields. This richness of the straight coffee substrates however, make for higher chances of contamination.

Coffee grounds are better used as asupplementadded to other substrate materials, such as hardwood sawdust or coco-coir. Coffee grounds should be fully sterilized before being added to the substrate, but you can often get away with just pasteurization.

Uses for Spent Mushroom Substrate

If you start to grow a lot of mushrooms, you might soon end up with large piles of spent substrate that you don’t know what to do with. The shear volume of substrate can add up really quick- so it’s good to have some idea of what to do with it all.

The most obvious choice, if you have a reasonably small amount of spent substrate, is to compost it. Just leave your substrate in a pile outside and let the natural process of decomposition take hold. Chances are good that you might even get an extra flush or two of mushrooms in your compost pile! It doesn’t take long before your spent substrate will decompose into a rich loamy compost that can be added to your vegetable garden.

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It is also possible that substrate can be reused to grow more mushrooms. For example, if you have a bunch of spent shiitake blocks, you might be able to break them up, re-sterilize them and reuse the organic material to grow more shiitake! Keep in mind though, you will likely need to add supplementation, and you are likely to have yields with diminishing returns.

If you have more spent substrate then you know what to do with, see if your city has a free composting program, or if a nearby farmer is willing to let you start a large compost pile on their land.

Many large commercial mushroom farming operations actually process and sell their spent mushroom substrate as branded compost for gardens.

Learn What Works for You

At the end of the day, what substrate is best for you will depend on your location, style of growing and type of mushrooms you want to grow.

The best way to learn is to experiment with different types of substrates, different methods of preparation and different types of supplementation.

Good luck!

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Posted by

Tony Shields

Hi' I'm Tony. I've dedicated a ton of time to learn all about the amazing world of mushrooms- and would love to share that information with you! I am particularily passionate about growing mushrooms, and teaching others how to do the same.

Understanding Mushroom Substrates - FreshCap Mushrooms (2024)


Understanding Mushroom Substrates - FreshCap Mushrooms? ›

A good substrate is required in order for the mushroom to grow and to fruit. Common substrate recipes for growing mushrooms usually involve straw or hardwood sawdust, although there are many other good options to be considered.

Which substrate for which mushroom? ›

Substrate Suitability for Growing Mushrooms
Mushroom StrainGrowing MethodCompatible Substrate
Almond agaricusGarden beds, containersManure, high-nitrogen compost
Lion's ManeLogs, containersLogs, straw, hardwood sawdust, wood chips
OysterLogs, containers, garden bedsHardwood sawdust, straw, wood chips, agricultural waste*
2 more rows

What is the best substrate for enoki mushrooms? ›

Enoki mushrooms prefer hardwood substrates, and you'll get the highest yields from supplemented sawdust or master's mix. But like oyster mushrooms, they'll grow on various organic substrates, including cardboard, straw and coffee grounds.

What are the steps in mushroom substrate? ›

  1. STEP 1: Growing out a chosen mushroom culture on an agar filled petri dish.
  2. STEP 2: Transferring the mushroom culture (known as mycelium) onto sterilized grain.
  3. STEP 3: Expanding the grain jar exponentially to create grain spawn.
  4. STEP 4: Spawning the grain into a suitable substrate and fruiting container.

What is the best ratio for mushroom substrate? ›

The ideal ratio for a bulk mushroom substrate using coco coir is 1 part coco coir with 1 part vermiculite. Start by pasteurizing the mixture, combining equal parts of coco coir and vermiculite.

What is the bulk substrate for cubensis? ›


Manure is the aged, dried excrement of horses, cows, elephants, etc. It is one of the most effective bulk substrates for dung loving species like psilocybe cubensis, panaeolus cyanescens and agaricus bisporus (Portobello). It is usually cheap or free if it can be located.

What is the easiest mushroom substrate? ›

Pretty much every mushroom growing resource I could find says that oyster mushrooms are the easiest variety for first time-growers, as they grow fast and can easily thrive in substrates made of things like coffee grounds and straw, making them relatively low maintenance.

Is rice a good substrate for mushrooms? ›

The substrates with 25 and 50% of raw or carbonized rice husk in a mixture with sawdust provided nitrogen and water more adequately to the fungus, improving the fresh mass production, the protein content of mushrooms, and biological efficiency (75–83%) compared to the reference substrate (100% eucalyptus sawdust), ...

What is the preferred substrate for shiitake mushrooms? ›

Shiitake mushrooms are typically grown on logs, but many growers are now using sawdust as the preferred substrate. The reason for this is that bags of sawdust are easier to deal with than logs of wood. A mix of hardwood sawdust with 5% wheat bran is an excellent substrate for shiitake mushrooms.

What is the best substrate for golden oyster mushrooms? ›

Oyster mushrooms can grow on a variety of things including wood logs, cottonseed hulls, corn cobs or office paper, but in North America, cereal straws are the substrate of choice (wheat and oat straw do particularly well). Equipment is fairly simple and outstanding results are obtained relatively quickly.

Why add gypsum to mushroom substrate? ›

Gypsum has been found to shorten the time it takes for the mycelium to grow throughout the substrate, according to researchers at the University of Putra Malaysia. That means it's quicker for the spores to grow throughout the growing block and start to grow mushrooms.

Can you reuse mushroom substrate? ›

Yes, you can most definitely reuse mushroom grow kits! Mushroom grow kits are generally designed to give you more than one harvest depending on the species of mushroom inside your growing kit. There is enough water nutrition available in the substrate to give you multiple harvests over a period of 2-10 weeks.

How long does it take for mushroom substrate to colonize? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

Are coffee grounds a good mushroom substrate? ›

It just makes sense to grow mushrooms in coffee grounds. You make wonderful nutritious oyster mushrooms out of a bountiful waste resource that is still rich with nutrients. You can return the now composted grounds to enrich your soil at the end of the growth cycle to complete its life cycle too.

How much coffee grounds to add to mushroom substrate? ›

Coffee grounds should comprise 77% of your substrate's total weight. Pasteurized straw or sawdust equal to 15% of the total substrate weight or 20% of the weight of the coffee grounds. Oyster or sh*take mushroom spawn equal to 8% of the total substrate weight or 10% of the weight of coffee grounds.

What happens if mushroom substrate is too dry? ›

However, excessive moisture can cause the substrate to become very wet, increasing mold growth. On the other hand, low humidity can cause the substrate to dry out, leading to poor mushroom spore and fruiting development.

What substrate for mushroom farming? ›

Straw is the commonest substrate for Oyster Mushroom cultivation. Other agricultural wastes (e.g. corn stalks) can be used. Invasive plant species such as water hyacinth and kudzu are also possible substrates.

What is the best substrate for mushroom bags? ›

Common Mushroom Substrates

Hardwoods such as oak, beech and maple make for a great substrate for many types of mushrooms, especially when combined with a bran supplement.

What mushroom substrate for Lion's Mane? ›

The best types of substrate to grow your Lion's Mane on include: Hardwood sawdust amended with bran. A Master's mix of 50-50 hardwood sawdust and soy hulls.

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